prestained protein marker x  (Servicebio Inc)

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Chidamide alters histone acetylation, cell cycle regulators, and mitochondrial apoptosis in SCLC cells. ( A, D, G ) H69, ( B, E, H ) H526, and ( C, F, I ) H446 cells were treated with Chidamide at indicated concentrations or DMSO control for 48 h. Western blot analysis demonstrated dose-dependent decrease in HDAC1/2/3, increase in Ac-H3 and Ac-H4, downregulation of Cyclin E1 and CDK2, upregulation of p21 and p27, and activation of mitochondrial apoptosis via altered Bcl-2 and Bax expression. GAPDH was used as loading control. Data represent three independent experiments

Article Snippet: Samples (50 μg) were separated by SDS-PAGE (Epizyme Biotech), transferred to PVDF membranes (Millipore), blocked with 5% skim milk, and incubated overnight at 4 °C with primary antibodies (1:1000, Cell Signaling Technology) against HDAC1–3, histones (H3, Ac-H3, Ac-H4), DNA damage/repair markers (γ-H2AX, p21, p27), cell cycle regulators (Cyclin E1, CDK2), apoptosis-related proteins (BCL2, BAX, Cytochrome C, PARP, Cleaved-PARP, Caspase-3, Cleaved caspase-3), and GAPDH (1:5000, Proteintech).

Techniques: Control, Western Blot, Activation Assay, Expressing

Journal: Discover Oncology

Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes

doi: 10.1007/s12672-025-04356-4

Figure Lengend Snippet: Immunohistochemical and Western blot analyses of histone acetylation, DNA damage markers, and apoptosis-related proteins in Chidamide-treated SCLC xenografts. A–C Immunohistochemical (IHC) staining of formalin-fixed paraffin-embedded tumor sections from H69, H526, and H446 xenografts treated with vehicle (Control), low-dose (12.5 mg/kg), and high-dose (25 mg/kg) Chidamide, assessing Ac-H3, γ-H2AX, p21, and Cleaved caspase-3 expression (Scale bar: 20 μm). D–F Quantification of IHC staining intensity (mean optical density ± SD, n = 3 independent experiments) using Image-Pro Plus software. G Western blot analysis of tumor lysates for H3, Ac-H3, γ-H2AX, p21, Caspase-3, and Cleaved caspase-3. GAPDH served as a loading control. Statistical analysis was performed using GraphPad Prism 5 with two-way ANOVA followed by the Bonferroni test (* P < 0.05, ** P < 0.01, *** P < 0.001, ns )

Article Snippet: Samples (50 μg) were separated by SDS-PAGE (Epizyme Biotech), transferred to PVDF membranes (Millipore), blocked with 5% skim milk, and incubated overnight at 4 °C with primary antibodies (1:1000, Cell Signaling Technology) against HDAC1–3, histones (H3, Ac-H3, Ac-H4), DNA damage/repair markers (γ-H2AX, p21, p27), cell cycle regulators (Cyclin E1, CDK2), apoptosis-related proteins (BCL2, BAX, Cytochrome C, PARP, Cleaved-PARP, Caspase-3, Cleaved caspase-3), and GAPDH (1:5000, Proteintech).

Techniques: Immunohistochemical staining, Western Blot, Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Control, Expressing, Software